Design

If you have any question or run into problems, please contact us and mention the job ID.
The job ID can be found in the URL and should look something like this: job_tJB1rv5w4pVh6YOftJXl

Paste your BED-file

Species

Primer Tm

min.

opt.

max.

Max. Tm diff

Primer %GC

min.

opt.

max.

Amplicon length

min.

max.

Auto determine

Design space (number of nucleotides on each side of the circRNA back-spliced junction)

SNP filter

'strict' avoids common SNPs in primer sequence; 'loose' avoids common SNPs in the 3' end half of the primer

Specificity filter

'strict' requires at least 3 mismatches in one primer and 2 mismatches in the other primer; 'loose' also allows 2 and 2 mismatches, or 2 and 1 mismatch

Spliced input sequence?